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CRISPR/Cas-mediated knockin in human pluripotent stem cells

Fluorescent reporter and epitope tagged human pluripotent stem cells (hPSCs) greatly facilitate studies on the pluripotency and differentiation characteristics of these cells. Unfortunately traditional procedures to generate such lines are hampered by a low targeting efficiency that necessitates a l...

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Bibliografische gegevens
Gepubliceerd in:Methods Mol Biol
Hoofdauteurs: Verma, Nipun, Zhu, Zengrong, Huangfu, Danwei
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2017
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5554709/
https://ncbi.nlm.nih.gov/pubmed/27807834
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6539-7_9
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