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CRISPR/Cas-mediated knockin in human pluripotent stem cells
Fluorescent reporter and epitope tagged human pluripotent stem cells (hPSCs) greatly facilitate studies on the pluripotency and differentiation characteristics of these cells. Unfortunately traditional procedures to generate such lines are hampered by a low targeting efficiency that necessitates a l...
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| Publicado no: | Methods Mol Biol |
|---|---|
| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5554709/ https://ncbi.nlm.nih.gov/pubmed/27807834 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6539-7_9 |
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