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CRISPR/Cas9-Mediated Mutagenesis of Human Pluripotent Stem Cells in Defined Xeno-free E8 Medium

The recent advent of engineered nucleases including the CRISPR/Cas9 system has greatly facilitated genome manipulation in human pluripotent stem cells (hPSCs). In addition to facilitating hPSC-based disease studies, the application of genome engineering in hPSCs has also opened up new avenues for ce...

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Bibliografiske detaljer
Udgivet i:Methods Mol Biol
Main Authors: Soh, Chew-Li, Huangfu, Danwei
Format: Artigo
Sprog:Inglês
Udgivet: 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5554712/
https://ncbi.nlm.nih.gov/pubmed/27709569
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6472-7_5
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