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A Versatile Approach to Site-specifically Install Lysine Acylations in Proteins
Using amber suppression in coordination with a mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl) pair, azidonorleucine is genetically encoded in E. coli. Its genetic incorporation followed by traceless Staudinger ligation with a phosphinothioester allows convenient synthesis of a protein with a site-speci...
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| 發表在: | Angew Chem Int Ed Engl |
|---|---|
| Main Authors: | , , , , , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2017
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5550319/ https://ncbi.nlm.nih.gov/pubmed/28042700 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201611415 |
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