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Construction of Allosteric Protein Switches by Alternate Frame Folding and Intermolecular Fragment Exchange

Alternate frame folding (AFF) and protein/fragment exchange (FREX) are related technologies for engineering allosteric conformational changes into proteins that have no pre-existing allosteric properties. One of their chief purposes is to turn an ordinary protein into a biomolecular switch capable o...

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Библиографические подробности
Опубликовано в: :Methods Mol Biol
Главные авторы: Ha, Jeung-Hoi, Loh, Stewart N.
Формат: Artigo
Язык:Inglês
Опубликовано: 2017
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5538016/
https://ncbi.nlm.nih.gov/pubmed/28293878
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6940-1_2
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