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Engineering an artificial zymogen by alternate frame protein folding
Alternate frame folding (AFF) is a novel mechanism by which allostery can be introduced into a protein where none may have existed previously. We employ this technology to convert the cytotoxic ribonuclease barnase into an artificial zymogen that is activated by HIV-1 protease. The AFF modification...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2840272/ https://ncbi.nlm.nih.gov/pubmed/20133757 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0907668107 |
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