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Modular Enzyme Design: Regulation by Mutually Exclusive Protein Folding

A regulatory mechanism is introduced whereupon the catalytic activity of a given enzyme is controlled by ligand binding to a receptor domain of choice. A small enzyme (barnase) and a ligand-binding polypeptide (GCN4) are fused so that a simple topological constraint prevents them from existing simul...

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Detalhes bibliográficos
Main Authors: Ha, Jeung-Hoi, Butler, James S., Mitrea, Diana M., Loh, Stewart N.
Formato: Artigo
Idioma:Inglês
Publicado em: 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3145369/
https://ncbi.nlm.nih.gov/pubmed/16483603
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2006.01.073
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