Efficient CRISPR-Cas9-mediated generation of knock-in human pluripotent stem cells lacking undesired mutations at the targeted locus

The CRISPR-Cas9 system has the potential to revolutionize genome editing in human pluripotent stem cells (hPSCs), but its advantages and pitfalls are still poorly understood. We systematically tested the ability of CRISPR-Cas9 to mediate reporter gene knock-in at 16 distinct genomic sites in hPSCs....

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Опубликовано в: :Cell Rep
Главные авторы: Merkle, Florian T., Neuhausser, Werner M., Santos, David, Valen, Eivind, Gagnon, James A., Maas, Kristi, Sandoe, Jackson, Schier, Alexander F., Eggan, Kevin
Формат: Artigo
Язык:Inglês
Опубликовано: 2015
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Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5533178/
https://ncbi.nlm.nih.gov/pubmed/25937281
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.celrep.2015.04.007
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