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Efficient CRISPR-Cas9-mediated generation of knock-in human pluripotent stem cells lacking undesired mutations at the targeted locus

The CRISPR-Cas9 system has the potential to revolutionize genome editing in human pluripotent stem cells (hPSCs), but its advantages and pitfalls are still poorly understood. We systematically tested the ability of CRISPR-Cas9 to mediate reporter gene knock-in at 16 distinct genomic sites in hPSCs....

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Detalhes bibliográficos
Publicado no:Cell Rep
Main Authors: Merkle, Florian T., Neuhausser, Werner M., Santos, David, Valen, Eivind, Gagnon, James A., Maas, Kristi, Sandoe, Jackson, Schier, Alexander F., Eggan, Kevin
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5533178/
https://ncbi.nlm.nih.gov/pubmed/25937281
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.celrep.2015.04.007
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