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Efficient CRISPR-Cas9-mediated generation of knock-in human pluripotent stem cells lacking undesired mutations at the targeted locus
The CRISPR-Cas9 system has the potential to revolutionize genome editing in human pluripotent stem cells (hPSCs), but its advantages and pitfalls are still poorly understood. We systematically tested the ability of CRISPR-Cas9 to mediate reporter gene knock-in at 16 distinct genomic sites in hPSCs....
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| 發表在: | Cell Rep |
|---|---|
| Main Authors: | , , , , , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2015
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5533178/ https://ncbi.nlm.nih.gov/pubmed/25937281 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.celrep.2015.04.007 |
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