CRISPR/Cas9 Genetic Modification of CYP3A5 *3 in HuH-7 Human Hepatocyte Cell Line Leads to Cell Lines with Increased Midazolam and Tacrolimus Metabolism

Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 engineering of the CYP3A5 *3 locus (rs776746) in human liver cell line HuH-7 (CYP3A5 *3/*3) has led to three CYP3A5 *1 cell lines by deletion of the exon 3B splice junction or point mutation. Cell lines CYP3A5 *1/*3 sd (single d...

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Publicado en:Drug Metab Dispos
Autores principales: Dorr, Casey R., Remmel, Rory P., Muthusamy, Amutha, Fisher, James, Moriarity, Branden S., Yasuda, Kazuto, Wu, Baolin, Guan, Weihua, Schuetz, Erin G., Oetting, William S., Jacobson, Pamala A., Israni, Ajay K.
Formato: Artigo
Lenguaje:Inglês
Publicado: The American Society for Pharmacology and Experimental Therapeutics 2017
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Articles
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5518718/
https://ncbi.nlm.nih.gov/pubmed/28533324
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1124/dmd.117.076307
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