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CRISPR–Cas9 Mediated DNA Unwinding Detected Using Site-Directed Spin Labeling

The RNA-guided CRISPR–Cas9 nuclease has revolutionized genome engineering, yet its mechanism for DNA target selection is not fully understood. A crucial step in Cas9 target recognition involves unwinding of the DNA duplex to form a three-stranded R-loop structure. Work reported here demonstrates dir...

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Detalhes bibliográficos
Publicado no:ACS Chem Biol
Main Authors: Tangprasertchai, Narin S., Di Felice, Rosa, Zhang, Xiaojun, Slaymaker, Ian M., Reyes, Carolina Vazquez, Jiang, Wei, Rohs, Remo, Qin, Peter Z.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5501335/
https://ncbi.nlm.nih.gov/pubmed/28437608
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acschembio.6b01137
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