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Kinetics are probe-dependent during downhill folding of an engineered λ(6–85) protein

The Y22W/Q33Y/G46,48A mutant of the protein λ(6–85) folds in a few microseconds at room temperature. We find that its folding kinetics are probe-dependent under a strong bias toward the native state, a new signature for downhill folding. The IR- and fluorescence-detected relaxation time scales conve...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Ma, Hairong, Gruebele, Martin
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2005
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Online Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC548978/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15699334/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.0409270102
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