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Using Fractional Intensities of Time-resolved Fluorescence to Sensitively Quantify NADH/NAD(+) with Genetically Encoded Fluorescent Biosensors
In this paper, we propose a novel and sensitive ratiometric analysis method that uses the fractional intensities of time-resolved fluorescence of genetically encoded fluorescent NADH/NAD(+) biosensors, Peredox, SoNar, and Frex. When the conformations of the biosensors change upon NADH/NAD(+) binding...
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| 出版年: | Sci Rep |
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| 主要な著者: | , , , , , , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Nature Publishing Group UK
2017
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5482812/ https://ncbi.nlm.nih.gov/pubmed/28646144 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-04051-7 |
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