Using Fractional Intensities of Time-resolved Fluorescence to Sensitively Quantify NADH/NAD(+) with Genetically Encoded Fluorescent Biosensors

In this paper, we propose a novel and sensitive ratiometric analysis method that uses the fractional intensities of time-resolved fluorescence of genetically encoded fluorescent NADH/NAD(+) biosensors, Peredox, SoNar, and Frex. When the conformations of the biosensors change upon NADH/NAD(+) binding...

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Publicat a:Sci Rep
Autors principals: Chang, Mengfang, Li, Lei, Hu, Hanyang, Hu, Qingxun, Wang, Aoxue, Cao, Xiaodan, Yu, Xiantong, Zhang, Sanjun, Zhao, Yuzheng, Chen, Jinquan, Yang, Yi, Xu, Jianhua
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group UK 2017
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5482812/
https://ncbi.nlm.nih.gov/pubmed/28646144
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-04051-7
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