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Methods for Using a Genetically Encoded Fluorescent Biosensor to Monitor Nuclear NAD(+)
Free nicotinamide adenine dinucleotide (NAD(+)) serves as substrate for NAD(+)-consuming enzymes. As such, the local concentration of free NAD(+) can influence enzymatic activities. Here we describe methods for using a fluorescent, genetically-encoded sensor to measure subcellular NAD(+) concentrati...
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| Pubblicato in: | Methods Mol Biol |
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| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2018
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6378224/ https://ncbi.nlm.nih.gov/pubmed/30097882 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-8588-3_26 |
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