Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins

BACKGROUND: Conditional knockout mice and transgenic mice expressing recombinases, reporters, and inducible transcriptional activators are key for many genetic studies and comprise over 90% of mouse models created. Conditional knockout mice are generated using labor-intensive methods of homologous r...

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Published in:Genome Biol
Main Authors: Quadros, Rolen M., Miura, Hiromi, Harms, Donald W., Akatsuka, Hisako, Sato, Takehito, Aida, Tomomi, Redder, Ronald, Richardson, Guy P., Inagaki, Yutaka, Sakai, Daisuke, Buckley, Shannon M., Seshacharyulu, Parthasarathy, Batra, Surinder K., Behlke, Mark A., Zeiner, Sarah A., Jacobi, Ashley M., Izu, Yayoi, Thoreson, Wallace B., Urness, Lisa D., Mansour, Suzanne L., Ohtsuka, Masato, Gurumurthy, Channabasavaiah B.
Format: Artigo
Language:Inglês
Published: BioMed Central 2017
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Research
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5434640/
https://ncbi.nlm.nih.gov/pubmed/28511701
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-017-1220-4
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