Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins

BACKGROUND: Conditional knockout mice and transgenic mice expressing recombinases, reporters, and inducible transcriptional activators are key for many genetic studies and comprise over 90% of mouse models created. Conditional knockout mice are generated using labor-intensive methods of homologous r...

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Detalhes bibliográficos
Publicado no:Genome Biol
Main Authors: Quadros, Rolen M., Miura, Hiromi, Harms, Donald W., Akatsuka, Hisako, Sato, Takehito, Aida, Tomomi, Redder, Ronald, Richardson, Guy P., Inagaki, Yutaka, Sakai, Daisuke, Buckley, Shannon M., Seshacharyulu, Parthasarathy, Batra, Surinder K., Behlke, Mark A., Zeiner, Sarah A., Jacobi, Ashley M., Izu, Yayoi, Thoreson, Wallace B., Urness, Lisa D., Mansour, Suzanne L., Ohtsuka, Masato, Gurumurthy, Channabasavaiah B.
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2017
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5434640/
https://ncbi.nlm.nih.gov/pubmed/28511701
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-017-1220-4
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