Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens

CRISPR-Cas9 screens are powerful tools for high-throughput interrogation of genome function, but can be confounded by nuclease-induced toxicity at both on- and off-target sites, likely due to DNA damage. Here, to test potential solutions to this issue, we design and analyse a CRISPR-Cas9 library wit...

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Publicado no:Nat Commun
Main Authors: Morgens, David W., Wainberg, Michael, Boyle, Evan A., Ursu, Oana, Araya, Carlos L., Tsui, C. Kimberly, Haney, Michael S., Hess, Gaelen T., Han, Kyuho, Jeng, Edwin E., Li, Amy, Snyder, Michael P., Greenleaf, William J., Kundaje, Anshul, Bassik, Michael C.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5424143/
https://ncbi.nlm.nih.gov/pubmed/28474669
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms15178
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