Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens

CRISPR-Cas9 screens are powerful tools for high-throughput interrogation of genome function, but can be confounded by nuclease-induced toxicity at both on- and off-target sites, likely due to DNA damage. Here, to test potential solutions to this issue, we design and analyse a CRISPR-Cas9 library wit...

詳細記述

保存先:
書誌詳細
出版年:Nat Commun
主要な著者: Morgens, David W., Wainberg, Michael, Boyle, Evan A., Ursu, Oana, Araya, Carlos L., Tsui, C. Kimberly, Haney, Michael S., Hess, Gaelen T., Han, Kyuho, Jeng, Edwin E., Li, Amy, Snyder, Michael P., Greenleaf, William J., Kundaje, Anshul, Bassik, Michael C.
フォーマット: Artigo
言語:Inglês
出版事項: Nature Publishing Group 2017
主題:
Article
オンライン･アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC5424143/
https://ncbi.nlm.nih.gov/pubmed/28474669
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms15178
タグ: タグ追加
タグなし, このレコードへの初めてのタグを付けませんか!

類似資料