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Broad substrate tolerance of tubulin tyrosine ligase enables one-step site-specific enzymatic protein labeling

The broad substrate tolerance of tubulin tyrosine ligase is the basic rationale behind its wide applicability for chemoenzymatic protein functionalization. In this context, we report that the wild-type enzyme enables ligation of various unnatural amino acids that are substantially bigger than and st...

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Vydáno v:Chem Sci
Hlavní autoři: Schumacher, Dominik, Lemke, Oliver, Helma, Jonas, Gerszonowicz, Lena, Waller, Verena, Stoschek, Tina, Durkin, Patrick M., Budisa, Nediljko, Leonhardt, Heinrich, Keller, Bettina G., Hackenberger, Christian P. R.
Médium: Artigo
Jazyk:Inglês
Vydáno: Royal Society of Chemistry 2017
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5418632/
https://ncbi.nlm.nih.gov/pubmed/28507719
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c7sc00574a
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