Impact of posttranslational modifications of engineered cysteines on the substituted cysteine accessibility method: evidence for glutathionylation

The substituted cysteine accessibility method (SCAM) is widely used to study the structure and function of channels, receptors and transporters. In its usual application, a cysteine residue is introduced into a protein which lacks native cysteines following which the accessibility of the residue to...

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Veröffentlicht in:Am J Physiol Cell Physiol
Hauptverfasser: Zhao, Rongbao, Najmi, Mitra, Aluri, Srinivas, Goldman, I. David
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Physiological Society 2017
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Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5407019/
https://ncbi.nlm.nih.gov/pubmed/28122733
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1152/ajpcell.00350.2016
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