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Structural and functional characterization of a ubiquitin variant engineered for tight and specific binding to an alpha‐helical ubiquitin interacting motif

Ubiquitin interacting motifs (UIMs) are short α‐helices found in a number of eukaryotic proteins. UIMs interact weakly but specifically with ubiquitin conjugated to other proteins, and in so doing, mediate specific cellular signals. Here we used phage display to generate ubiquitin variants (UbVs) ta...

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Detalles Bibliográficos
Publicado en:Protein Sci
Main Authors: Manczyk, Noah, Yates, Bradley P., Veggiani, Gianluca, Ernst, Andreas, Sicheri, Frank, Sidhu, Sachdev S.
Formato: Artigo
Idioma:Inglês
Publicado: John Wiley and Sons Inc. 2017
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5405428/
https://ncbi.nlm.nih.gov/pubmed/28276594
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3155
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