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Structural and functional characterization of a ubiquitin variant engineered for tight and specific binding to an alpha‐helical ubiquitin interacting motif

Ubiquitin interacting motifs (UIMs) are short α‐helices found in a number of eukaryotic proteins. UIMs interact weakly but specifically with ubiquitin conjugated to other proteins, and in so doing, mediate specific cellular signals. Here we used phage display to generate ubiquitin variants (UbVs) ta...

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Detaylı Bibliyografya
Yayımlandı:Protein Sci
Asıl Yazarlar: Manczyk, Noah, Yates, Bradley P., Veggiani, Gianluca, Ernst, Andreas, Sicheri, Frank, Sidhu, Sachdev S.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: John Wiley and Sons Inc. 2017
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC5405428/
https://ncbi.nlm.nih.gov/pubmed/28276594
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3155
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