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Substrate Recognition and Modification by a Pathogen-Associated Aminoglycoside Resistance 16S rRNA Methyltransferase

The pathogen-associated 16S rRNA methyltransferase NpmA catalyzes m(1)A1408 modification to block the action of structurally diverse aminoglycoside antibiotics. Here, we describe the development of a fluorescence polarization binding assay and its use, together with complementary functional assays,...

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Detalhes bibliográficos
Publicado no:Antimicrob Agents Chemother
Main Authors: Vinal, Kellie, Conn, Graeme L.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5404524/
https://ncbi.nlm.nih.gov/pubmed/28289026
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.00077-17
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