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Simultaneous detection of mRNA transcription and decay intermediates by dual colour single mRNA FISH on subcellular resolution

The detection of mRNAs undergoing transcription or decay is challenging, because both processes are fast. However, the relative proportion of an mRNA in synthesis or decay increases with mRNA size and decreases with mRNA half-life. Based on this rationale, I have exploited a 22 200 nucleotide-long,...

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Détails bibliographiques
Publié dans:Nucleic Acids Res
Auteur principal: Kramer, Susanne
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2017
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5397161/
https://ncbi.nlm.nih.gov/pubmed/27940558
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw1245
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