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Simultaneous detection of mRNA transcription and decay intermediates by dual colour single mRNA FISH on subcellular resolution

The detection of mRNAs undergoing transcription or decay is challenging, because both processes are fast. However, the relative proportion of an mRNA in synthesis or decay increases with mRNA size and decreases with mRNA half-life. Based on this rationale, I have exploited a 22 200 nucleotide-long,...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Autor principal: Kramer, Susanne
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5397161/
https://ncbi.nlm.nih.gov/pubmed/27940558
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw1245
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