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Simultaneous detection of mRNA transcription and decay intermediates by dual colour single mRNA FISH on subcellular resolution

The detection of mRNAs undergoing transcription or decay is challenging, because both processes are fast. However, the relative proportion of an mRNA in synthesis or decay increases with mRNA size and decreases with mRNA half-life. Based on this rationale, I have exploited a 22 200 nucleotide-long,...

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Vydáno v:Nucleic Acids Res
Hlavní autor: Kramer, Susanne
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2017
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5397161/
https://ncbi.nlm.nih.gov/pubmed/27940558
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw1245
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