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Simultaneous detection of the subcellular localization of RNAs and proteins in cultured cells by combined multicolor RNA-FISH and IF
Fluorescence in situ hybridization (FISH) and immunofluorescence (IF) are sensitive techniques for detecting nucleic acids and proteins in cultured cells. However, these techniques are rarely applied together, and standard protocols are not readily compatible for sequential application on the same s...
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| Veröffentlicht in: | Methods |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2016
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5360554/ https://ncbi.nlm.nih.gov/pubmed/27664292 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2016.09.010 |
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