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Simultaneous detection of the subcellular localization of RNAs and proteins in cultured cells by combined multicolor RNA-FISH and IF

Fluorescence in situ hybridization (FISH) and immunofluorescence (IF) are sensitive techniques for detecting nucleic acids and proteins in cultured cells. However, these techniques are rarely applied together, and standard protocols are not readily compatible for sequential application on the same s...

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Detalhes bibliográficos
Publicado no:Methods
Main Authors: Meyer, Cindy, Garzia, Aitor, Tuschl, Thomas
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5360554/
https://ncbi.nlm.nih.gov/pubmed/27664292
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2016.09.010
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