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Optimization of PAR-CLIP for transcriptome-wide identification of binding sites of RNA-binding proteins

Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP) in combination with next-generation sequencing is a powerful method for identifying endogenous targets of RNA-binding proteins (RBPs). Depending on the characteristics of each RBP, key steps in the PAR-CLIP proc...

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Publicat a:Methods
Autors principals: Garzia, Aitor, Meyer, Cindy, Morozov, Pavel, Sajek, Marcin, Tuschl, Thomas
Format: Artigo
Idioma:Inglês
Publicat: 2016
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5393971/
https://ncbi.nlm.nih.gov/pubmed/27765618
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2016.10.007
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