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Optimization of PAR-CLIP for transcriptome-wide identification of binding sites of RNA-binding proteins
Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP) in combination with next-generation sequencing is a powerful method for identifying endogenous targets of RNA-binding proteins (RBPs). Depending on the characteristics of each RBP, key steps in the PAR-CLIP proc...
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| Publicado en: | Methods |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2016
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5393971/ https://ncbi.nlm.nih.gov/pubmed/27765618 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2016.10.007 |
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