PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation): a Step-By-Step Protocol to the Transcriptome-Wide Identification of Binding Sites of RNA-Binding Proteins

We recently developed a protocol for the transcriptome-wide isolation of RNA recognition elements readily applicable to any protein or ribonucleoprotein complex directly contacting RNA (including RNA helicases, polymerases, or nucleases) expressed in cell culture models either naturally or ectopical...

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Detalhes bibliográficos
Main Authors: Spitzer, Jessica, Hafner, Markus, Landthaler, Markus, Ascano, Manuel, Farazi, Thalia, Wardle, Greg, Nusbaum, Jeff, Khorshid, Mohsen, Burger, Lukas, Zavolan, Mihaela, Tuschl, Thomas
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4180672/
https://ncbi.nlm.nih.gov/pubmed/24581442
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-420120-0.00008-6
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