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CLIP: Crosslinking and ImmunoPrecipitation of In Vivo RNA Targets of RNA-Binding Proteins

We present a newly developed method for fixing RNA–protein complexes in situ in living cells and the subsequent purification of the RNA targets. Using this approach, complex tissue such as mouse brain can be ultraviolet (UV) irradiated to covalently crosslink RNA–protein complexes. Once covalently b...

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Publicat a:Methods Mol Biol
Autors principals: Jensen, Kirk B., Darnell, Robert B.
Format: Artigo
Idioma:Inglês
Publicat: 2008
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4492529/
https://ncbi.nlm.nih.gov/pubmed/18982285
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60327-475-3_6
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