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CLIP: Crosslinking and ImmunoPrecipitation of In Vivo RNA Targets of RNA-Binding Proteins
We present a newly developed method for fixing RNA–protein complexes in situ in living cells and the subsequent purification of the RNA targets. Using this approach, complex tissue such as mouse brain can be ultraviolet (UV) irradiated to covalently crosslink RNA–protein complexes. Once covalently b...
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| Publié dans: | Methods Mol Biol |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2008
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4492529/ https://ncbi.nlm.nih.gov/pubmed/18982285 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60327-475-3_6 |
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