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Pooled CRISPR screening with single-cell transcriptome read-out
CRISPR-based genetic screens are accelerating biological discovery, but current methods have inherent limitations. Widely used pooled screens are restricted to simple readouts including cell proliferation and sortable marker proteins. Arrayed screens allow for comprehensive molecular readouts such a...
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| Veröffentlicht in: | Nat Methods |
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| Hauptverfasser: | , , , , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2017
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5334791/ https://ncbi.nlm.nih.gov/pubmed/28099430 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4177 |
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