Genome-wide barcoded transposon screen for cancer drug sensitivity in haploid mouse embryonic stem cells

We describe a screen for cellular response to drugs that makes use of haploid embryonic stem cells. We generated ten libraries of mutants with piggyBac gene trap transposon integrations, totalling approximately 100,000 mutant clones. Random barcode sequences were inserted into the transposon vector...

Popoln opis

Shranjeno v:
Bibliografske podrobnosti
izdano v:Sci Data
Main Authors: Pettitt, Stephen J., Krastev, Dragomir B., Pemberton, Helen N., Fontebasso, Yari, Frankum, Jessica, Rehman, Farah L., Brough, Rachel, Song, Feifei, Bajrami, Ilirjana, Rafiq, Rumana, Wallberg, Fredrik, Kozarewa, Iwanka, Fenwick, Kerry, Armisen-Garrido, Javier, Swain, Amanda, Gulati, Aditi, Campbell, James, Ashworth, Alan, Lord, Christopher J.
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group 2017
Teme:
Data Descriptor
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5332012/
https://ncbi.nlm.nih.gov/pubmed/28248920
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sdata.2017.20
Oznake: Označite
Brez oznak, prvi označite!

Podobne knjige/članki