Genome-wide barcoded transposon screen for cancer drug sensitivity in haploid mouse embryonic stem cells

We describe a screen for cellular response to drugs that makes use of haploid embryonic stem cells. We generated ten libraries of mutants with piggyBac gene trap transposon integrations, totalling approximately 100,000 mutant clones. Random barcode sequences were inserted into the transposon vector...

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Veröffentlicht in:Sci Data
Hauptverfasser: Pettitt, Stephen J., Krastev, Dragomir B., Pemberton, Helen N., Fontebasso, Yari, Frankum, Jessica, Rehman, Farah L., Brough, Rachel, Song, Feifei, Bajrami, Ilirjana, Rafiq, Rumana, Wallberg, Fredrik, Kozarewa, Iwanka, Fenwick, Kerry, Armisen-Garrido, Javier, Swain, Amanda, Gulati, Aditi, Campbell, James, Ashworth, Alan, Lord, Christopher J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group 2017
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5332012/
https://ncbi.nlm.nih.gov/pubmed/28248920
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sdata.2017.20
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