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A divalent cation-dependent variant of the glmS ribozyme with stringent Ca(2+) selectivity co-opts a preexisting nonspecific metal ion-binding site
Ribozymes use divalent cations for structural stabilization, as catalytic cofactors, or both. Because of the prominent role of Ca(2+) in intracellular signaling, engineered ribozymes with stringent Ca(2+) selectivity would be important in biotechnology. The wild-type glmS ribozyme (glmS(WT)) require...
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| Publicado no: | RNA |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5311495/ https://ncbi.nlm.nih.gov/pubmed/27932587 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.059824.116 |
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