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A divalent cation-dependent variant of the glmS ribozyme with stringent Ca(2+) selectivity co-opts a preexisting nonspecific metal ion-binding site

Ribozymes use divalent cations for structural stabilization, as catalytic cofactors, or both. Because of the prominent role of Ca(2+) in intracellular signaling, engineered ribozymes with stringent Ca(2+) selectivity would be important in biotechnology. The wild-type glmS ribozyme (glmS(WT)) require...

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Publicado en:RNA
Main Authors: Lau, Matthew W.L., Trachman, Robert J., Ferré-D'Amaré, Adrian R.
Formato: Artigo
Idioma:Inglês
Publicado: Cold Spring Harbor Laboratory Press 2017
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5311495/
https://ncbi.nlm.nih.gov/pubmed/27932587
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.059824.116
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