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In vitro evolution of coenzyme-independent variants from the glmS ribozyme structural scaffold
Uniquely among known natural ribozymes that cleave RNA sequence-specifically, the glmS ribozyme-riboswitch employs a small molecule, glucosamine-6-phosphate (GlcN6P) as a catalytic cofactor. In vitro selection was employed to search for coenzyme-independent variants of this ribozyme. In addition to...
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| Veröffentlicht in: | Methods |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2016
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4981508/ https://ncbi.nlm.nih.gov/pubmed/27130889 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2016.04.027 |
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