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Topoisomerase I‐mediated cleavage at unrepaired ribonucleotides generates DNA double‐strand breaks

Ribonuclease activity of topoisomerase I (Top1) causes DNA nicks bearing 2′,3′‐cyclic phosphates at ribonucleotide sites. Here, we provide genetic and biochemical evidence that DNA double‐strand breaks (DSBs) can be directly generated by Top1 at sites of genomic ribonucleotides. We show that RNase H...

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Bibliografische gegevens
Gepubliceerd in:EMBO J
Hoofdauteurs: Huang, Shar‐yin N, Williams, Jessica S, Arana, Mercedes E, Kunkel, Thomas A, Pommier, Yves
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: John Wiley and Sons Inc. 2016
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5286372/
https://ncbi.nlm.nih.gov/pubmed/27932446
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15252/embj.201592426
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