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Conversion of Topoisomerase I Cleavage Complexes on the Leading Strand of Ribosomal DNA into 5′-Phosphorylated DNA Double-Strand Breaks by Replication Runoff

Topoisomerase I cleavage complexes can be induced by a variety of DNA damages and by the anticancer drug camptothecin. We have developed a ligation-mediated PCR (LM-PCR) assay to analyze replication-mediated DNA double-strand breaks induced by topoisomerase I cleavage complexes in human colon carcin...

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Detalhes bibliográficos
Main Authors: Strumberg, Dirk, Pilon, André A., Smith, Melanie, Hickey, Robert, Malkas, Linda, Pommier, Yves
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2000
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC85758/
https://ncbi.nlm.nih.gov/pubmed/10805740
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