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Genome-scale RNA interference screen identifies antizyme 1(OAZ1) as a target for improvement of recombinant protein production in mammalian cells()

For the purpose of improving recombinant protein production from mammalian cells, an unbiased, high-throughput whole-genome RNA interference screen was conducted using human embryonic kidney 293 (HEK 293) cells expressing firefly luciferase. 21,585 human genes were individually silenced with three d...

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Vydáno v:Biotechnol Bioeng
Hlavní autoři: Xiao, Su, Chen, Yu Chi, Buehler, Eugene, Mandal, Swati, Mandal, Ajeet, Betenbaugh, Michael, HeePark, Myung, Martin, Scott, Shiloach, Joseph
Médium: Artigo
Jazyk:Inglês
Vydáno: 2016
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5283837/
https://ncbi.nlm.nih.gov/pubmed/27215166
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.26017
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