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Measuring the sequence-affinity landscape of antibodies with massively parallel titration curves

Despite the central role that antibodies play in the adaptive immune system and in biotechnology, much remains unknown about the quantitative relationship between an antibody’s amino acid sequence and its antigen binding affinity. Here we describe a new experimental approach, called Tite-Seq, that i...

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Detalles Bibliográficos
Publicado en:eLife
Main Authors: Adams, Rhys M, Mora, Thierry, Walczak, Aleksandra M, Kinney, Justin B
Formato: Artigo
Idioma:Inglês
Publicado: eLife Sciences Publications, Ltd 2016
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5268739/
https://ncbi.nlm.nih.gov/pubmed/28035901
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.23156
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