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Titration-free massively parallel pyrosequencing using trace amounts of starting material
Continuous efforts have been made to improve next-generation sequencing methods for increased robustness and for applications on low amounts of starting material. We applied double-stranded library protocols for the Roche 454 platform to avoid the yield-reducing steps associated with single-stranded...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2910068/ https://ncbi.nlm.nih.gov/pubmed/20435675 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq332 |
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