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Titration-free massively parallel pyrosequencing using trace amounts of starting material

Continuous efforts have been made to improve next-generation sequencing methods for increased robustness and for applications on low amounts of starting material. We applied double-stranded library protocols for the Roche 454 platform to avoid the yield-reducing steps associated with single-stranded...

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Détails bibliographiques
Auteurs principaux: Zheng, Zongli, Advani, Abdolreza, Melefors, Öjar, Glavas, Steve, Nordström, Henrik, Ye, Weimin, Engstrand, Lars, Andersson, Anders F.
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2010
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2910068/
https://ncbi.nlm.nih.gov/pubmed/20435675
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq332
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