Accuracy and quality of massively parallel DNA pyrosequencing

BACKGROUND: Massively parallel pyrosequencing systems have increased the efficiency of DNA sequencing, although the published per-base accuracy of a Roche GS20 is only 96%. In genome projects, highly redundant consensus assemblies can compensate for sequencing errors. In contrast, studies of microbi...

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Detalhes bibliográficos
Main Authors: Huse, Susan M, Huber, Julie A, Morrison, Hilary G, Sogin, Mitchell L, Welch, David Mark
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2007
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2323236/
https://ncbi.nlm.nih.gov/pubmed/17659080
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/gb-2007-8-7-r143
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