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In Vitro Reconstruction of Nonribosomal Peptide Biosynthesis Directly from DNA Using Cell-Free Protein Synthesis

Genome sequencing has revealed that a far greater number of natural product biosynthetic pathways exist than there are known natural products. To access these molecules directly and deterministically, a new generation of heterologous expression methods is needed. Cell-free protein synthesis has not...

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Veröffentlicht in:ACS Synth Biol
Hauptverfasser: Goering, Anthony W., Li, Jian, McClure, Ryan A., Thomson, Regan J., Jewett, Michael C., Kelleher, Neil L.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2016
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5250536/
https://ncbi.nlm.nih.gov/pubmed/27478992
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.6b00160
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