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Kinetic and Regiospecific Interrogation of Covalent Intermediates in the Nonribosomal Peptide Synthesis of Yersiniabactin
For interrogation of enzyme-bound intermediates in nonribosomal peptide synthetases (NRPSs), mass spectrometry is used to read out the kinetics and substrate specificity of this medicinally important class of enzymes. The protein HMWP2 (230 kDa) catalyzes 11 chemical reactions, four of which could b...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1307228/ https://ncbi.nlm.nih.gov/pubmed/15479080 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja0470867 |
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