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Comparison of SHAPE reagents for mapping RNA structures inside living cells

Recent advances in SHAPE technology have converted the classic primer extension method to next-generation sequencing platforms, allowing transcriptome-level analysis of RNA secondary structure. In particular, icSHAPE and SHAPE-MaP, using NAI-N(3) and 1M7 reagents, respectively, are methods that clai...

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Detalhes bibliográficos
Publicado no:RNA
Main Authors: Lee, Byron, Flynn, Ryan A., Kadina, Anastasia, Guo, Jimmy K., Kool, Eric T., Chang, Howard Y.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5238792/
https://ncbi.nlm.nih.gov/pubmed/27879433
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.058784.116
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